This book summarizes the research contents, general process flow and learning methods of bioseparation and purification technology. It takes the basic content of bioseparation and purification technology as the main line, it is arranged into chapters to expound cytoclasis, sediment, extraction, filtration and membrane separation, chromatographic separation. The rationale, basic operation and typical equipment of bioseparation and purification technology like concentration, drying, crystallization and others and the specific use in the biotechnology, and introduces appropriately relate new knowledge, new technology and new methods.