Practical HPLC Method Development

Table of contents:getting startedbasics of separationdetection sensitivity and selectivitysample preparationthe columnnon-ionic samples: reversed and normal-phase hplcionic samples: reversed-phase, ion-pair and ion-exchange hplcgradient elutionsystematic approach to the reversed-phase separation of regular samplescomputer-assisted method developmentbiochemical samples: proteins, nucleic acids, carbohydrates and related compoundschiral separationspreparative hplc separationquantitation (including trace analysis)completing the method: validation and transferappendicesindex