Optimizing utilization of DNA from rare or archival anthropological samples.: An article from: Human Biology
Book Details
PublisherWayne State University Press
ISBN / ASINB00092XQXW
ISBN-13978B00092XQX3
AvailabilityAvailable for download now
MarketplaceUnited States 🇺🇸
Description
This digital document is an article from Human Biology, published by Wayne State University Press on October 1, 1994. The length of the article is 5903 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser.
From the author: There is widespread interest in obtaining genetic samples from human populations worldwide for various studies of human genetic diversity. Many samples exist today only in the form of small, rare, irreplaceable, or archival samples, such as material from ancient bone, hair bulbs, or remnants of samples collected in the field decades ago for the purpose of protein and blood type analysis. Here, we describe the application of an approach to amplify DNA, which we call adapter attachment and amplification (AAA). This approach is useful for amplifying the genome in a reasonably representative way from small amounts of starting material using standard PCR-based methods. We apply a version of these methods to DNA extracted from washed red blood cells collected in the 1960s and 1970s in the Amazon basin. AAA and similar approaches may make the analysis of archival samples possible without exhausting that irreplaceable material and may lead to greatly improved efficiency in collecting and using new anthropological genetic samples.
Citation Details
Title: Optimizing utilization of DNA from rare or archival anthropological samples.
Author: Kenneth M. Weiss
Publication:Human Biology (Refereed)
Date: October 1, 1994
Publisher: Wayne State University Press
Volume: v66 Issue: n5 Page: p789(16)
Distributed by Thomson Gale
From the author: There is widespread interest in obtaining genetic samples from human populations worldwide for various studies of human genetic diversity. Many samples exist today only in the form of small, rare, irreplaceable, or archival samples, such as material from ancient bone, hair bulbs, or remnants of samples collected in the field decades ago for the purpose of protein and blood type analysis. Here, we describe the application of an approach to amplify DNA, which we call adapter attachment and amplification (AAA). This approach is useful for amplifying the genome in a reasonably representative way from small amounts of starting material using standard PCR-based methods. We apply a version of these methods to DNA extracted from washed red blood cells collected in the 1960s and 1970s in the Amazon basin. AAA and similar approaches may make the analysis of archival samples possible without exhausting that irreplaceable material and may lead to greatly improved efficiency in collecting and using new anthropological genetic samples.
Citation Details
Title: Optimizing utilization of DNA from rare or archival anthropological samples.
Author: Kenneth M. Weiss
Publication:Human Biology (Refereed)
Date: October 1, 1994
Publisher: Wayne State University Press
Volume: v66 Issue: n5 Page: p789(16)
Distributed by Thomson Gale
