Molecular systematic studies in cycads: evidence from trnL intron and ITS2 rDNA sequences.: An article from: The Botanical Review
Book Details
Author(s)
David J. Bogler, Javier Francisco-Ortega
Publisher
Thomson Gale
ISBN / ASIN
B000EHT6PO
ISBN-13
978B000EHT6P6
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Available for download now
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#14,455,473
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This digital document is an article from The Botanical Review, published by Thomson Gale on April 1, 2004. The length of the article is 6542 words. The page length shown above is based on a typical 300-word page. The article is delivered in HTML format and is available in your Amazon.com Digital Locker immediately after purchase. You can view it with any web browser.
From the author: The results of a pilot DNA sequencing study of cycads conducted at the new molecular systematics laboratory at Fairchild Tropical Garden are presented and assessed with reference to previous phylogenetic analyses and classification schemes based on morphology and anatomy. Two DNA regions were sequenced and analyzed for variation, an intron in the trnL gene in the chloroplast genome (trnL intron) and the internal transcribed spacer region between the 5.8S and 26S ribosomal DNA subunits (ITS2). The trnL intron proved to be relatively conservative among cycad genera, while the ITS2 region contained higher levels of variation. Parsimony analysis of the sequences suggests a number of relationships, some of which were inferred by previous morphological studies, some of which are new. The sequences of Cycas are the most divergent among cycads, suggesting the longest isolation. Dioon is relatively isolated from the other genera and contains two major clades. Stangeria does not appear closely related to Bowenia but does seem to have a weak affinity with Zamia and Microcycas. Lepidozamia is more closely related to Encephalartos than to Macrozamia. Sequence variation among the species of Ceratozamia is low. Microcycas and Zamia are closely related.
Citation Details
Title: Molecular systematic studies in cycads: evidence from trnL intron and ITS2 rDNA sequences.
Author: David J. Bogler
Publication:The Botanical Review (Magazine/Journal)
Date: April 1, 2004
Publisher: Thomson Gale
Volume: 70 Issue: 2 Page: 260(14)
Distributed by Thomson Gale
From the author: The results of a pilot DNA sequencing study of cycads conducted at the new molecular systematics laboratory at Fairchild Tropical Garden are presented and assessed with reference to previous phylogenetic analyses and classification schemes based on morphology and anatomy. Two DNA regions were sequenced and analyzed for variation, an intron in the trnL gene in the chloroplast genome (trnL intron) and the internal transcribed spacer region between the 5.8S and 26S ribosomal DNA subunits (ITS2). The trnL intron proved to be relatively conservative among cycad genera, while the ITS2 region contained higher levels of variation. Parsimony analysis of the sequences suggests a number of relationships, some of which were inferred by previous morphological studies, some of which are new. The sequences of Cycas are the most divergent among cycads, suggesting the longest isolation. Dioon is relatively isolated from the other genera and contains two major clades. Stangeria does not appear closely related to Bowenia but does seem to have a weak affinity with Zamia and Microcycas. Lepidozamia is more closely related to Encephalartos than to Macrozamia. Sequence variation among the species of Ceratozamia is low. Microcycas and Zamia are closely related.
Citation Details
Title: Molecular systematic studies in cycads: evidence from trnL intron and ITS2 rDNA sequences.
Author: David J. Bogler
Publication:The Botanical Review (Magazine/Journal)
Date: April 1, 2004
Publisher: Thomson Gale
Volume: 70 Issue: 2 Page: 260(14)
Distributed by Thomson Gale
