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Remazol Brilliant Blue R decolourization by the laccase from Trametes [An article from: Chemosphere]
Book Details
Author(s)T. Mechichi, N. Mhiri, S. Sayadi
PublisherElsevier
ISBN / ASINB000P6O6LS
ISBN-13978B000P6O6L0
MarketplaceUnited Kingdom 🇬🇧
Description
This digital document is a journal article from Chemosphere, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
The decolourization of the recalcitrant dye RBBR by the culture filtrate of Trametes trogii and its isolated laccase was investigated. Both filtrates from Cu-induced cultures as well as purified laccase decolourized the dye RBBR. The purified laccase decolourized the dye down to 97% of 100mgl^-^1 initial concentration of RBBR when only 0.2Uml^-^1 of laccase was used in the reaction mixture. The effects of different physicochemical parameters were tested and optimal decolourization rates occurred at pH 5 and at a temperature of 50^oC. Decolourization of RBBR occurred in the presence of metal ions which could be found in textile industry effluents. Of all the metal ions tested, FeCl"2 was the most inhibiting for the decolourization. HBT was shown to have no effect on the decolourization of RBBR at low concentration, while at a concentration of 5mM it slightly inhibited decolourization. The presence of aromatic compounds was found to be inhibiting for the decolourization at a concentration of 10mM, but not at 0.1mM, while at 1mM only ortho-diphenols were inhibiting. Probing the effect of methanol it was found that higher concentrations caused a decrease in the decolourization rate of RBBR. The effect of laccase inhibitors on the decolourization of RBBR was tested with l-cysteine, SDS and EDTA. It was demonstrated that l-cysteine was the most inhibiting substrate for the decolourization while SDS was only inhibiting at 10mM concentration and ETDA was not inhibiting at all tested concentrations.
Description:
The decolourization of the recalcitrant dye RBBR by the culture filtrate of Trametes trogii and its isolated laccase was investigated. Both filtrates from Cu-induced cultures as well as purified laccase decolourized the dye RBBR. The purified laccase decolourized the dye down to 97% of 100mgl^-^1 initial concentration of RBBR when only 0.2Uml^-^1 of laccase was used in the reaction mixture. The effects of different physicochemical parameters were tested and optimal decolourization rates occurred at pH 5 and at a temperature of 50^oC. Decolourization of RBBR occurred in the presence of metal ions which could be found in textile industry effluents. Of all the metal ions tested, FeCl"2 was the most inhibiting for the decolourization. HBT was shown to have no effect on the decolourization of RBBR at low concentration, while at a concentration of 5mM it slightly inhibited decolourization. The presence of aromatic compounds was found to be inhibiting for the decolourization at a concentration of 10mM, but not at 0.1mM, while at 1mM only ortho-diphenols were inhibiting. Probing the effect of methanol it was found that higher concentrations caused a decrease in the decolourization rate of RBBR. The effect of laccase inhibitors on the decolourization of RBBR was tested with l-cysteine, SDS and EDTA. It was demonstrated that l-cysteine was the most inhibiting substrate for the decolourization while SDS was only inhibiting at 10mM concentration and ETDA was not inhibiting at all tested concentrations.
