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Separation of proteases from yellowfin tuna spleen by ultrafiltration [An article from: Bioresource Technology]
Book Details
Author(s)Z. Li, W. Youravong, A. H-Kittikun
PublisherElsevier
ISBN / ASINB000P6OCU8
ISBN-13978B000P6OCU6
MarketplaceFrance 🇫🇷
Description
This digital document is a journal article from Bioresource Technology, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Separation of protease, trypsin and chymotrypsin from yellowfin tuna spleen extract by ultrafiltration (UF) using regenerated cellulose membranes with molecular weight cut off (MWCO) 30 and 100kDa was studied. The 100kDa membrane had a higher transmission of enzymes than that of the 30kDa membrane. The enzyme transmission varied from 0.01 to 0.18 and from 0.6 to 0.8 for the 30kDa membrane and 100kDa membrane, respectively. The protein transmission was about 0.8 for both membranes. Increasing cross-flow rate and transmembrane pressure (TMP) increased permeate flux. The limiting fluxes at cross-flow rate 120, 240 and 360L/h for the 30kDa membrane were 17.3, 43.9 and 54.7L/m^2h, respectively and the limiting fluxes at the same flow rate for 100 kDa membrane were 34.1, 51.1 and 68.4L/m^2h, respectively. The separation of these proteases was achieved using the 30kDa membrane. The purities of proteases were increased more than ten times at TMP 1.5bar and cross-flow rate 360L/h by diafiltration using 30kDa membrane.
Description:
Separation of protease, trypsin and chymotrypsin from yellowfin tuna spleen extract by ultrafiltration (UF) using regenerated cellulose membranes with molecular weight cut off (MWCO) 30 and 100kDa was studied. The 100kDa membrane had a higher transmission of enzymes than that of the 30kDa membrane. The enzyme transmission varied from 0.01 to 0.18 and from 0.6 to 0.8 for the 30kDa membrane and 100kDa membrane, respectively. The protein transmission was about 0.8 for both membranes. Increasing cross-flow rate and transmembrane pressure (TMP) increased permeate flux. The limiting fluxes at cross-flow rate 120, 240 and 360L/h for the 30kDa membrane were 17.3, 43.9 and 54.7L/m^2h, respectively and the limiting fluxes at the same flow rate for 100 kDa membrane were 34.1, 51.1 and 68.4L/m^2h, respectively. The separation of these proteases was achieved using the 30kDa membrane. The purities of proteases were increased more than ten times at TMP 1.5bar and cross-flow rate 360L/h by diafiltration using 30kDa membrane.
