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Determination of biogenic amines in apples and wine with 8-phenyl-(4-oxy-acetic acid N-hydroxysuccinimide ester)-4, ... [An article from: Analytica Chimica Acta]
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This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
A precolumn-derivatization high performance liquid chromatographic method for biogenic amine analysis has been developed. Derivatization of biogenic amines was performed with a new fluorescent reagent, 8-phenyl-(4-oxy-acetic acid N-hydroxysuccinimide ester)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene (TMPAB-OSu), which was synthesized in our lab. The derivatization conditions and the influence of elution composition on the separation were investigated. In pH 8.5 H"3BO"3-Na"2B"4O"7 media, the reaction of biogenic amines with TMPAB-OSu completed at 45^oC for 5min. The labeled biogenic amines were separated on an ZORBAX Eclipse XDB-C"8 column (150mmx4.6mm, 5@mm) and detected with fluorescence at excitation and emission wavelengths of 497 and 509nm, respectively. Detection limits of biogenic amines were 0.1-4nmolL^-^1, at a signal-to-noise ratio of 3. The proposed method has been applied to the quantitative determination of spermine, phenethylamine, spermidine, cadaverine and putrescine in pericarp and pulp of mature and immature apples and wine with recoveries of 95-102% and R.S.D. of 0.7-3.9.
Description:
A precolumn-derivatization high performance liquid chromatographic method for biogenic amine analysis has been developed. Derivatization of biogenic amines was performed with a new fluorescent reagent, 8-phenyl-(4-oxy-acetic acid N-hydroxysuccinimide ester)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene (TMPAB-OSu), which was synthesized in our lab. The derivatization conditions and the influence of elution composition on the separation were investigated. In pH 8.5 H"3BO"3-Na"2B"4O"7 media, the reaction of biogenic amines with TMPAB-OSu completed at 45^oC for 5min. The labeled biogenic amines were separated on an ZORBAX Eclipse XDB-C"8 column (150mmx4.6mm, 5@mm) and detected with fluorescence at excitation and emission wavelengths of 497 and 509nm, respectively. Detection limits of biogenic amines were 0.1-4nmolL^-^1, at a signal-to-noise ratio of 3. The proposed method has been applied to the quantitative determination of spermine, phenethylamine, spermidine, cadaverine and putrescine in pericarp and pulp of mature and immature apples and wine with recoveries of 95-102% and R.S.D. of 0.7-3.9.
