![Analysis of protease digestion patterns in tideland sediments using SDS-PAGE [An article from: Journal of Experimental Marine Biology and Ecology]](https://www.ebooknetworking.net/books/B00/0P6/bigB000P6OWXA.jpg)
Analysis of protease digestion patterns in tideland sediments using SDS-PAGE [An article from: Journal of Experimental Marine Biology and Ecology]
Price not available for France
You can still browse on Amazon. Try another country above.
Book Details
Author(s)
K.i. Nakamura
Publisher
Elsevier
ISBN / ASIN
B000P6OWXA
ISBN-13
978B000P6OWX6
Marketplace
France 🇫🇷
Ratings & Reviews
No reviews yet — be the first!
No reviews yet.
Description
This digital document is a journal article from Journal of Experimental Marine Biology and Ecology, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
A method to analyse protein hydrolysis in tideland sediments was developed using sodium lauryl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE). Bovine serum albumin was used as a substrate and sonication in SDS solution was employed to extract hydrolysed peptides from tideland sediments. By combining these techniques, digested peptides were fractionated reliably on slab gels by SDS-PAGE. Using this method, the protease activities in a tideland were found to be distributed only in the sediment fraction and not in the seawater. The protease activities in sediments were compared among three tidelands in the Seto Inland Sea. Niho tideland, the most polluted of the three tidelands examined, showed the highest levels of protease activity. Peptide cleavage patterns were very similar among the tidelands despite the differences in levels of protease activity, suggesting that the tidelands all contain common types of protease. Furthermore, the properties of proteases in tideland sediments were also characterised using the present method.
Description:
A method to analyse protein hydrolysis in tideland sediments was developed using sodium lauryl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE). Bovine serum albumin was used as a substrate and sonication in SDS solution was employed to extract hydrolysed peptides from tideland sediments. By combining these techniques, digested peptides were fractionated reliably on slab gels by SDS-PAGE. Using this method, the protease activities in a tideland were found to be distributed only in the sediment fraction and not in the seawater. The protease activities in sediments were compared among three tidelands in the Seto Inland Sea. Niho tideland, the most polluted of the three tidelands examined, showed the highest levels of protease activity. Peptide cleavage patterns were very similar among the tidelands despite the differences in levels of protease activity, suggesting that the tidelands all contain common types of protease. Furthermore, the properties of proteases in tideland sediments were also characterised using the present method.
