![Construction of a genetically engineered microorganism for degrading organophosphate and carbamate pesticides [An article from: International Biodeterioration & Biodegradation]](https://www.ebooknetworking.net/books/B00/0PB/bigB000PBZRFC.jpg)
Construction of a genetically engineered microorganism for degrading organophosphate and carbamate pesticides [An article from: International Biodeterioration & Biodegradation]
Description
This digital document is a journal article from International Biodeterioration & Biodegradation, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Genetically engineered microorganisms (GEMs) have shown potential for enhanced degradation of a range of substrates. In this study, a DNA fragment including the open reading frame of mpd (methyl parathion hydrolase encoding gene) and cognate regulator of a methyl parathion (MP)-degrading strain Pseudomonas putida DLL-1 was cloned by the shotgun method. The fragment was cloned into a broad-host vector pBBR1MCS-2 to produce a recombinant plasmid pBBR-mpd. CDS-pBBR-mpd, a GEM, was successfully constructed by transforming it into a carbofuran-degrading strain Sphingomonas sp. CDS-1, and it degraded both carbofuran and MP. CDS-pBBR-mpd maintained stable and high MP hydrolase activity (50.72nmol min^-^1 @mg^-^1 protein, which was 6.57 times higher than that of P. putida strain DLL-1). and appears to be a promising GEM for environmental bioremediation.
Description:
Genetically engineered microorganisms (GEMs) have shown potential for enhanced degradation of a range of substrates. In this study, a DNA fragment including the open reading frame of mpd (methyl parathion hydrolase encoding gene) and cognate regulator of a methyl parathion (MP)-degrading strain Pseudomonas putida DLL-1 was cloned by the shotgun method. The fragment was cloned into a broad-host vector pBBR1MCS-2 to produce a recombinant plasmid pBBR-mpd. CDS-pBBR-mpd, a GEM, was successfully constructed by transforming it into a carbofuran-degrading strain Sphingomonas sp. CDS-1, and it degraded both carbofuran and MP. CDS-pBBR-mpd maintained stable and high MP hydrolase activity (50.72nmol min^-^1 @mg^-^1 protein, which was 6.57 times higher than that of P. putida strain DLL-1). and appears to be a promising GEM for environmental bioremediation.
