Synthesis of silica-based benzeneboronic acid affinity materials and application as pre-column in coupled-column high-performance liquid chromatography [An article from: Analytica Chimica Acta]

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Description:
Three silica-based benzeneboronic acid affinity materials were synthesized by using an m-aminobenzeneboronic acid as the ligand and using three different spacer arms. Under high-pressure, three affinity pre-columns were packed with these materials and the retention of every affinity pre-column with 11 urinary nucleosides was studied. With different spacer arms of boronic acid-substituted silica materials, the absorption to vicinal alcohols (cis-diols) and stability under acidic elution conditions are of great difference. Coupled-column liquid chromatographic methods for the direct analysis of urinary nucleosides were respectively established. As a result, two of three affinity pre-columns showed good chromatographic property in the on-line analysis of urinary nucleosides. The coupled-column system including pre-column I is the best with excellent linearity (R^2>0.995), good recoveries (85.6-96.9%) and reproducibility (R.S.D.: 1.01-4.02%). The pre-column I could at least endure 150 repetitive injections of a 100@mL urinary sample.