![Use of vitamin B"2 for fluorescence detection of thymidine-related single-nucleotide polymorphisms [An article from: Analytica Chimica Acta]](https://www.ebooknetworking.net/books/B00/0PB/bigB000PBZYQE.jpg)
Use of vitamin B"2 for fluorescence detection of thymidine-related single-nucleotide polymorphisms [An article from: Analytica Chimica Acta]
Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
In combination with abasic site (AP site)-containing DNAs, potential use of a biotic fluorescence compound, Vitamin B"2 (riboflavin), is demonstrated for the fluorescence detection of the thymine (T)-related single-nucleotide polymorphisms. Our method is based on construction of the AP site in DNA duplexes, which allows small ligands to bind to target nucleotides accompanied by fluorescence signaling: an AP site-containing probe DNA is hybridized with a target DNA so as to place the AP site toward a target nucleobase, by which hydrophobic microenvironments are provided for ligands to recognize target nucleotides through stacking and hydrogen-bonding interactions. In 10mM sodium cacodylate buffer solutions (pH 7.0) containing 100mM NaCl and 1.0mM EDTA, Vitamin B"2 is found to selectively bind to T (K"1"1=1.8x10^6M^-^1 at 5^oC) over other nucleobases, and this is accompanied by significant quenching of its fluorescence. While the sensing functions depend on the flanking sequences to the AP site, Vitamin B"2 is applicable to the detection of T/C (cytosine), T/G (guanine) and T/A (adenine) mutation sequences of the CYP2A6 gene, where the flanking nucleobases are guanines in both positions (-GXG-, X=AP site).
Description:
In combination with abasic site (AP site)-containing DNAs, potential use of a biotic fluorescence compound, Vitamin B"2 (riboflavin), is demonstrated for the fluorescence detection of the thymine (T)-related single-nucleotide polymorphisms. Our method is based on construction of the AP site in DNA duplexes, which allows small ligands to bind to target nucleotides accompanied by fluorescence signaling: an AP site-containing probe DNA is hybridized with a target DNA so as to place the AP site toward a target nucleobase, by which hydrophobic microenvironments are provided for ligands to recognize target nucleotides through stacking and hydrogen-bonding interactions. In 10mM sodium cacodylate buffer solutions (pH 7.0) containing 100mM NaCl and 1.0mM EDTA, Vitamin B"2 is found to selectively bind to T (K"1"1=1.8x10^6M^-^1 at 5^oC) over other nucleobases, and this is accompanied by significant quenching of its fluorescence. While the sensing functions depend on the flanking sequences to the AP site, Vitamin B"2 is applicable to the detection of T/C (cytosine), T/G (guanine) and T/A (adenine) mutation sequences of the CYP2A6 gene, where the flanking nucleobases are guanines in both positions (-GXG-, X=AP site).
