![Sensitive and simple liquid chromatographic method with ultraviolet detection for the determination of nifedipine in canine plasma [An article from: Analytica Chimica Acta]](https://www.ebooknetworking.net/books/B00/0PD/bigB000PDT8PA.jpg)
Sensitive and simple liquid chromatographic method with ultraviolet detection for the determination of nifedipine in canine plasma [An article from: Analytica Chimica Acta]
Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
An isocratic high-performance liquid chromatographic method with detection at 240nm was developed, optimized and validated for the determination of nifedipine in canine plasma. Liquid-liquid extraction was used as the sample preparation technique. Carbamazepine was used as internal standard. A Hypersil BDS RP-C"1"8 column (250mmx4.6mm, 5@mm) was equilibrated with a mobile phase composed of water and methanol, 45:55 (v/v). Its flow rate was 1mlmin^-^1. The elution time for nifedipine and carbamazepine was approximately 12 and 8min, respectively. Calibration curves of nifedipine in plasma were linear in the concentration range of 1-200ngml^-^1. Limits of detection and quantification in plasma were 0.5 and 1.5ngml^-^1, respectively. Recovery was greater than 98%. Intra- and inter-day relative standard deviation for nifedipine in plasma was less than 8.5 and 10%, respectively. This method was applied to the determination of nifedipine plasma levels after administration of commercially available soft gelatine capsules to dogs.
Description:
An isocratic high-performance liquid chromatographic method with detection at 240nm was developed, optimized and validated for the determination of nifedipine in canine plasma. Liquid-liquid extraction was used as the sample preparation technique. Carbamazepine was used as internal standard. A Hypersil BDS RP-C"1"8 column (250mmx4.6mm, 5@mm) was equilibrated with a mobile phase composed of water and methanol, 45:55 (v/v). Its flow rate was 1mlmin^-^1. The elution time for nifedipine and carbamazepine was approximately 12 and 8min, respectively. Calibration curves of nifedipine in plasma were linear in the concentration range of 1-200ngml^-^1. Limits of detection and quantification in plasma were 0.5 and 1.5ngml^-^1, respectively. Recovery was greater than 98%. Intra- and inter-day relative standard deviation for nifedipine in plasma was less than 8.5 and 10%, respectively. This method was applied to the determination of nifedipine plasma levels after administration of commercially available soft gelatine capsules to dogs.
