Modelling flow rate gradient elution in reversed-phase liquid chromatography [An article from: Analytica Chimica Acta]

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Description:
The fundamental equation of flow programming elution was tested in several different types of flow-rate gradients (step, linear, multilinear, parabolic and more combined gradients) implemented in the separation of two multicomponent mixtures of solutes. The retention prediction obtained for all solutes under all flow-programmed conditions was excellent. In addition, although flow programming appears quite limited in its ability to provide improved performance in liquid chromatography, there are specific flow-rate profiles which provide significant improvement in the rearrangement of the peaks within a chromatogram.