![Using on-line solid phase extraction for determination of amiloride in human urine by sequential injection technique [An article from: Analytica Chimica Acta]](https://www.ebooknetworking.net/books/B00/0PD/bigB000PDT8SM.jpg)
Using on-line solid phase extraction for determination of amiloride in human urine by sequential injection technique [An article from: Analytica Chimica Acta]
Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
This presented paper deals with a new methodology for the direct determination of amiloride in human urine. The methodology described is based on the sequential injection analysis technique (SIA) coupled with solid phase extraction (SPE) microcolumn. SPE microcolumn was used for selective retention of amiloride, while the urine matrix components were eluted with water carrier flow to the waste. Due to the acid-basic and polarity properties of amiloride molecule and principles of ion-exchange chromatography, it was possible to retain amiloride on the ion-exchange sorbent (SPE BAKER WCX-carboxy group). Eluting solution was 0.01M HNO"3+0.1M KCl, flow rate 20@mls^-^1. The fluorescence detection of amiloride was performed at @l"e"m 385nm (secondary filter). Recovery was found in the range 96.8-99.4% for 10 times diluted urine, linearity of determination in the range 0.5-100@mgml^-^1 (r=0.998), and 3@s limit of detection (LOD) was 0.05@mgml^-^1. The whole procedure comprising raw sample pre-treatment, analyte detection and column reconditioning took 8min. The proposed SIA-SPE method has been applied for direct determination of amiloride in human urine.
Description:
This presented paper deals with a new methodology for the direct determination of amiloride in human urine. The methodology described is based on the sequential injection analysis technique (SIA) coupled with solid phase extraction (SPE) microcolumn. SPE microcolumn was used for selective retention of amiloride, while the urine matrix components were eluted with water carrier flow to the waste. Due to the acid-basic and polarity properties of amiloride molecule and principles of ion-exchange chromatography, it was possible to retain amiloride on the ion-exchange sorbent (SPE BAKER WCX-carboxy group). Eluting solution was 0.01M HNO"3+0.1M KCl, flow rate 20@mls^-^1. The fluorescence detection of amiloride was performed at @l"e"m 385nm (secondary filter). Recovery was found in the range 96.8-99.4% for 10 times diluted urine, linearity of determination in the range 0.5-100@mgml^-^1 (r=0.998), and 3@s limit of detection (LOD) was 0.05@mgml^-^1. The whole procedure comprising raw sample pre-treatment, analyte detection and column reconditioning took 8min. The proposed SIA-SPE method has been applied for direct determination of amiloride in human urine.
