Production of ethanol from starch by free and immobilized Candida tropicalis in the presence of @a-amylase [An article from: Bioresource Technology]

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Description:
Candida tropicalis is a potentially useful organism for the commercial production of ethanol as it is capable of fermenting starch at a low rate. To enhance this carbon source utilization and increase the rate of alcohol production, we pretreated corn soluble starch with @a-amylase. Starch liquefaction was sufficient to drive the fermentation and to convert 96% substrate to ethanol. Indeed, in the presence of exogenous @a-amylase, 9% (w/v) soluble starch was converted to 43.1g ethanol/l in 65h with a productivity of 0.65g/l h. Thus, bio-ethanol production using free and calcium alginate-immobilized C. tropicalis does not require the saccharification step. Furthermore, fed-batch fermentation by free C. tropicalis cells increased the final concentration to 56g ethanol/l, reaching published values for Saccharomyces cerevisiae recombinant strains expressing both @a-amylase and glucoamylase.