Biocatalytic esterification of @b-methylglucoside for synthesis of biocompatible sugar-containing vinyl esters [An article from: Chemical Engineering Journal]

This digital document is a journal article from Chemical Engineering Journal, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
Biocatalytic synthesis of @b-methylglucoside methacrylate/acrylate by the esterification of @b-methylglucoside with vinyl methacrylate/acrylate was carried out using an immobilized lipase from Candida antarctica (Novozym 435) in organic solvents. Among five model solvents including t-butanol, t-amyl alcohol, acetone, acetonitrile and 1,4-dioxane, t-butanol was found to be an optimal solvent. The amount of 5% (w/v) Novozym 435 resulted in the highest conversion after 48h, and 1:3 molar ratio of @b-methylglucoside to vinyl methacrylate showed 100% final conversion after 24h. The optimal initial concentration of @b-methylglucoside and temperature determined from the initial rate and final conversion were observed at 60g/l and 50^oC, respectively. The productivity of sugar esters could be enhanced by the heterogeneous reaction mixture and the addition of @b-methylglucoside after one cycle of reaction in homogeneous reaction. Based on the over 95% of cell viability by MTT assay, produced sugar esters were found to be highly biocompatible. Structural analysis of products was performed by FT-IR and ^1^3C NMR.