![Analysis of NAD(P)^+ and NAD(P)H cofactors by means of imprinted polymers associated with Au surfaces: [An article from: Analytica Chimica Acta]](https://www.ebooknetworking.net/books/B00/0RR/bigB000RR008S.jpg)
Analysis of NAD(P)^+ and NAD(P)H cofactors by means of imprinted polymers associated with Au surfaces: [An article from: Analytica Chimica Acta]
Book Details
PublisherElsevier
ISBN / ASINB000RR008S
ISBN-13978B000RR0088
AvailabilityAvailable for download now
MarketplaceUnited States 🇺🇸
Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Crosslinked films consisting of the acrylamide-acrylamidophenylboronic acid copolymer that are imprinted with recognition sites for @b-nicotinamide adenine dinucleotide (NAD^+), @b-nicotinamide adenine dinucleotide phosphate NADP^+, and their reduced forms (NAD(P)H), are assembled on Au-coated glass supports. The binding of the oxidized cofactors NAD^+ or NADP^+ or the reduced cofactors NADH or NADPH to the respective imprinted sites results in the swelling of the polymer films through the uptake of water. Surface plasmon resonance (SPR) spectroscopy is employed to follow the binding of the different cofactors to the respective imprinted sites. The imprinted recognition sites reveal selectivity towards the association of the imprinted cofactors. The method enables the analysis of the NAD(P)^+ and NAD(P)H cofactors in the concentration range of 1x10^-^6 to 1x10^-^3M. The cofactor-imprinted films associated with the Au-coated glass supports act as active interfaces for the characterization of biocatalyzed transformations that involve the cofactor-dependent enzymes. This is exemplified with the characterization of the biocatalyzed oxidation of lactate to pyruvate in the presence of NAD^+ and lactate dehydrogenase using the NADH-imprinted polymer film.
Description:
Crosslinked films consisting of the acrylamide-acrylamidophenylboronic acid copolymer that are imprinted with recognition sites for @b-nicotinamide adenine dinucleotide (NAD^+), @b-nicotinamide adenine dinucleotide phosphate NADP^+, and their reduced forms (NAD(P)H), are assembled on Au-coated glass supports. The binding of the oxidized cofactors NAD^+ or NADP^+ or the reduced cofactors NADH or NADPH to the respective imprinted sites results in the swelling of the polymer films through the uptake of water. Surface plasmon resonance (SPR) spectroscopy is employed to follow the binding of the different cofactors to the respective imprinted sites. The imprinted recognition sites reveal selectivity towards the association of the imprinted cofactors. The method enables the analysis of the NAD(P)^+ and NAD(P)H cofactors in the concentration range of 1x10^-^6 to 1x10^-^3M. The cofactor-imprinted films associated with the Au-coated glass supports act as active interfaces for the characterization of biocatalyzed transformations that involve the cofactor-dependent enzymes. This is exemplified with the characterization of the biocatalyzed oxidation of lactate to pyruvate in the presence of NAD^+ and lactate dehydrogenase using the NADH-imprinted polymer film.
