Multiresidue analysis of anabolic compounds in bovine hair by gas chromatography-tandem mass spectrometry [An article from: Analytica Chimica Acta]

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Description:
A rapid and specific multiresidue method was developed to perform screening and confirmation of 15 anabolics steroids in bovine hair, namely: dienestrol, DES, hexestrol, 17@a- and 17@b-estradiol, 17@a- and 17@b-19-nortestosterone, 17@a- and 17@b-boldenone, testosterone (@b) and epitestosterone (@a), ethynylestradiol, @a-methyltestosterone, and @a- and @b-Zearalanol. After methanolic and solid-phase extraction, trimethylsilyl derivatization (TMS) was performed and the derivatized extracts submitted to GC-MS-MS analysis. TMS derivatives of the studied hormones yielded specific MS-MS fragmentation with a high abundance for ions selected in the screening procedure. Validation was performed regarding qualitative parameters: decision limit (CC@a), detection capability (CC@b) and specificity. For confirmation, European union criteria 2002/657/EC were considered. Detection capability ranged between 1.0 and 10.0ngg^-^1 depending on the compound.