![Acetylcholinesterase sensors based on gold electrodes modified with dendrimer and polyaniline [An article from: Analytica Chimica Acta]](https://www.ebooknetworking.net/books/B00/0RR/bigB000RR02RC.jpg)
Acetylcholinesterase sensors based on gold electrodes modified with dendrimer and polyaniline [An article from: Analytica Chimica Acta]
Price not available for India
You can still browse on Amazon. Try another country above.
Book Details
Ratings & Reviews
No reviews yet — be the first!
No reviews yet.
Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Potentiometric and amperometric enzyme sensors based on modified gold electrodes have been developed and compared in pesticide determination. PAMAM dendrimer (generation G4) stabilized with 1-hexadecanethiol was used for the immobilization of acetylcholinesterase from electric eel and choline oxidase from Alcaligenes species in the assembly of amperometric sensor. Polyaniline-doped with camphorsulfonic acid was used to obtain potentiometric response. Trichlorfon, carbofuran and eserine suppress the biosensor response due to their inhibitory effect. The detection limits of 0.003 and 200nmoll^-^1 (trichlorfon), 0.04 and 6nmoll^-^1 (carbofuran) and 0.1 and 700nmoll^-^1 were obtained for amperometric and potentiometric sensors, respectively. The difference in the biosensor behavior and the high sensitivity of the dendrimer modified sensor to the inhibitors is due to the specific organization of protein layer at charged surface of the modifier macromolecules.
Description:
Potentiometric and amperometric enzyme sensors based on modified gold electrodes have been developed and compared in pesticide determination. PAMAM dendrimer (generation G4) stabilized with 1-hexadecanethiol was used for the immobilization of acetylcholinesterase from electric eel and choline oxidase from Alcaligenes species in the assembly of amperometric sensor. Polyaniline-doped with camphorsulfonic acid was used to obtain potentiometric response. Trichlorfon, carbofuran and eserine suppress the biosensor response due to their inhibitory effect. The detection limits of 0.003 and 200nmoll^-^1 (trichlorfon), 0.04 and 6nmoll^-^1 (carbofuran) and 0.1 and 700nmoll^-^1 were obtained for amperometric and potentiometric sensors, respectively. The difference in the biosensor behavior and the high sensitivity of the dendrimer modified sensor to the inhibitors is due to the specific organization of protein layer at charged surface of the modifier macromolecules.
