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A new approach for detection of antimicrobial drugs in food [An article from: Analytica Chimica Acta]
Book Details
PublisherElsevier
ISBN / ASINB000RR3G7K
ISBN-13978B000RR3G72
AvailabilityAvailable for download now
Sales Rank99,999,999
MarketplaceUnited States 🇺🇸
Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2005. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
A rapid, high-throughput antimicrobial screening assay has been developed that combines either a physical fluid extraction or a solvent extraction technique with the commercially available Premi^(R)Test. In order to remove the subjectivity of the visual end-point measurement associated with this microbial inhibition assay, work has been conducted to couple the Premi^(R)Test to scanner technology. The use of the solvent extraction provides an enhanced detection capability for a wide range of drugs at or below one-half the maximum residue limit (MRL) concentrations in a variety of matrices, as demonstrated by dose response curve data. Secondary class-specific assays, for the identification of @b-lactams and sulphonamides following the primary screen have been previously developed and recently validated using the scanner technology. Method validation using both fortified and incurred tissues has been undertaken to establish the ruggedness of the technique. The false-positive and -negative rates have been established at less than 5% for a range of drug/matrix combinations. The CC@b values determined for this qualitative screening assay are at concentrations less than the MRL. This integrated screening strategy provides a reliable tool for antimicrobial residue monitoring in surveillance programmes.
Description:
A rapid, high-throughput antimicrobial screening assay has been developed that combines either a physical fluid extraction or a solvent extraction technique with the commercially available Premi^(R)Test. In order to remove the subjectivity of the visual end-point measurement associated with this microbial inhibition assay, work has been conducted to couple the Premi^(R)Test to scanner technology. The use of the solvent extraction provides an enhanced detection capability for a wide range of drugs at or below one-half the maximum residue limit (MRL) concentrations in a variety of matrices, as demonstrated by dose response curve data. Secondary class-specific assays, for the identification of @b-lactams and sulphonamides following the primary screen have been previously developed and recently validated using the scanner technology. Method validation using both fortified and incurred tissues has been undertaken to establish the ruggedness of the technique. The false-positive and -negative rates have been established at less than 5% for a range of drug/matrix combinations. The CC@b values determined for this qualitative screening assay are at concentrations less than the MRL. This integrated screening strategy provides a reliable tool for antimicrobial residue monitoring in surveillance programmes.
