A method for the simultaneous determination of @b-ODAP, @a-ODAP, homoarginine and polyamines in Lathyrus sativus by liquid chromatography using a new ... [An article from: Analytica Chimica Acta]

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Description:
The paper proposes a new method to extract @b-N-oxalyl-l-@a,@b-diaminopropionic acid (@b-ODAP), @a-N-oxalyl-l-@a,@b-diaminopropionic acid (@a-ODAP), homoarginine and polyamines using 0.2M HClO"4 for only 1h at 0-4^oC. Based on the proposed extraction, an assay is presented for simultaneously determining polyamines, the neurotoxin @b-ODAP, its non-neurotoxic isomer @a-ODAP and homoarginine in Lathyrus sativus extracts using a liquid chromatography (LC) system after derivatization with para-nitrobenzyloxycarbonyl chloride (PNZ-Cl). The method exhibits some prominent advantages, which include: shorter extraction-time, simultaneous extraction of non-protein amino acids and polyamines and inhibition of the isomerization of @b-ODAP to @a-ODAP. The assay proved satisfactory for the quantitative determination of L. sativus extracts of seeds and seedlings.