![Structural identification of human blood phospholipids using liquid chromatography/quadrupole-linear ion trap mass spectrometry [An article from: Analytica Chimica Acta]](https://www.ebooknetworking.net/books/B00/0RR/bigB000RR3JL8.jpg)
Structural identification of human blood phospholipids using liquid chromatography/quadrupole-linear ion trap mass spectrometry [An article from: Analytica Chimica Acta]
Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
A normal-phase liquid chromatography/quadrupole-linear ion trap mass spectrometry method was developed for the separation and mass spectral characterization of the main phospholipid species in human blood. The instrument combines the capabilities of a triple quadrupole mass spectrometer and ion trap technology on a single platform. The optimal separation was achieved by using hexane/1-propanol as mobile phase and 0.6% formic acid, 0.06% ammonia as modifiers. The HPLC/MS technique was able to provide information about the molecular mass of individual homologues by positive and negative turbo ionspray. More complete characterization of fatty acid chains and of the polar head group was obtained by using a quadrupole collisionally activated dissociation (CAD) spectrum with ion trap sensitivity. The mass spectra and molecular species of phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylcholine (PC), lysophosphatidylcholine (lysoPC) and sphingomyelin (SM) are presented.
Description:
A normal-phase liquid chromatography/quadrupole-linear ion trap mass spectrometry method was developed for the separation and mass spectral characterization of the main phospholipid species in human blood. The instrument combines the capabilities of a triple quadrupole mass spectrometer and ion trap technology on a single platform. The optimal separation was achieved by using hexane/1-propanol as mobile phase and 0.6% formic acid, 0.06% ammonia as modifiers. The HPLC/MS technique was able to provide information about the molecular mass of individual homologues by positive and negative turbo ionspray. More complete characterization of fatty acid chains and of the polar head group was obtained by using a quadrupole collisionally activated dissociation (CAD) spectrum with ion trap sensitivity. The mass spectra and molecular species of phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylcholine (PC), lysophosphatidylcholine (lysoPC) and sphingomyelin (SM) are presented.
