Development of a liquid chromatography-tandem mass spectrometry method for the identification of natural androgen steroids and their conjugates in ... [An article from: Analytica Chimica Acta]

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Description:
An analytical procedure for the determination of natural steroids and their conjugates was developed and applied to bovine and human urine. Fifteen compounds (free and conjugated) after extraction by solid-phase were identified by liquid chromatography-tandem mass spectrometry (LC-MS-MS) in multiple reaction monitoring (MRM). Free and conjugated steroids were detected all together in the same chromatographic analysis respectively in positive and negative ionization. This procedure reveals to be time-saving, because it overcomes the need of further sample pre-treatment steps, such as enzymatic hydrolysis and derivatisation. The recovery for each compound was around 80%. Limit of detection of each steroid free or conjugated has been evaluated.