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Optimal growth conditions and the cultivation of Chaetoceros calcitrans in airlift photobioreactor [An article from: Chemical Engineering Journal]
Description
This digital document is a journal article from Chemical Engineering Journal, published by Elsevier in 2005. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
The optimal conditions for the growth of a diatom Chaetoceros calcitrans were investigated in a 2.5L glass bubble column. The light intensity for the highest growth rate was shown to be at around 400@mmolphotonsm^-^2s^-^1. A modified standard F/2 medium with a two-fold of silica and phosphorus concentrations was illustrated to result in a better growth of this diatom. Vitamin B"1"2 in the range from 1 to 3@mgL^-^1 did not significantly affect the growth. A maximum cell density obtained from this small glass bubble column was approximately 5.8x10^6cellsmL^-^1 with a maximum specific growth rate of 3.80x10^-^2h^-^1. The cultivation of C.calcitrans in the 17L airlift photobioreactor (ALPBR) was illustrated to be superior to that in the bubble column with the same size. The operation with superficial gas velocity of 3cms^-^1 was found to give a maximum specific growth rate of 7.41x10^-^2h^-^1 with a maximum cell concentration of 8.88x10^6cellsmL^-^1 in a batch culture. A semi-continuous culture could be achieved where the harvest was performed at every 12h. In this case, the maximum specific growth rate (@m) achievable was 9.65x10^-^2h^-^1 and the cell concentration during the harvest period was 4.08x10^6cellsmL^-^1.
Description:
The optimal conditions for the growth of a diatom Chaetoceros calcitrans were investigated in a 2.5L glass bubble column. The light intensity for the highest growth rate was shown to be at around 400@mmolphotonsm^-^2s^-^1. A modified standard F/2 medium with a two-fold of silica and phosphorus concentrations was illustrated to result in a better growth of this diatom. Vitamin B"1"2 in the range from 1 to 3@mgL^-^1 did not significantly affect the growth. A maximum cell density obtained from this small glass bubble column was approximately 5.8x10^6cellsmL^-^1 with a maximum specific growth rate of 3.80x10^-^2h^-^1. The cultivation of C.calcitrans in the 17L airlift photobioreactor (ALPBR) was illustrated to be superior to that in the bubble column with the same size. The operation with superficial gas velocity of 3cms^-^1 was found to give a maximum specific growth rate of 7.41x10^-^2h^-^1 with a maximum cell concentration of 8.88x10^6cellsmL^-^1 in a batch culture. A semi-continuous culture could be achieved where the harvest was performed at every 12h. In this case, the maximum specific growth rate (@m) achievable was 9.65x10^-^2h^-^1 and the cell concentration during the harvest period was 4.08x10^6cellsmL^-^1.
