![Acute actions of marine toxin latrunculin A on the electrophysiological properties of cultured dorsal root ganglion neurones [An article from: Comparative Biochemistry and Physiology, Part C]](https://www.ebooknetworking.net/books/B00/0RR/bigB000RR6JDS.jpg)
Acute actions of marine toxin latrunculin A on the electrophysiological properties of cultured dorsal root ganglion neurones [An article from: Comparative Biochemistry and Physiology, Part C]
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This digital document is a journal article from Comparative Biochemistry and Physiology, Part C, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
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The effects of latrunculin A, isolated from the nudibranch Chromodoris sp., on the excitability of neonatal rat cultured dorsal root ganglion neurones were investigated using patch-clamp recording and Ca^2^+ imaging techniques. Under current-clamp conditions, acute application of latrunculin A (100 @mM) reversibly induced multiple action potential firing and significantly increased action potential duration. No significant effects on action potential peak amplitude, threshold of action potential firing, resting membrane potential and input resistance were observed. Under voltage-clamp conditions, significant and dose-dependent suppression of K^+ current was seen with 10-100 @mM latrunculin A. Additionally, a significant difference between inhibition of the current measured at the peak and the end of a 100 ms voltage step was seen with 100 @mM latrunculin A. Fura-2 fluorescence Ca^2^+ imaging revealed that latrunculin A (100 @mM) significantly inhibited Ca^2^+ transients evoked by KCl-induced depolarisation in all neurones. In 36% of DRG neurones, latrunculin A alone had no effect on intracellular Ca^2^+. In 64% of neurones, latrunculin A alone evoked a transient rise in intracellular Ca^2^+. Moreover, latrunculin A (10-100 @mM) significantly inhibited the mean high voltage-activated Ca^2^+ current. The effects of latrunculin A on action potential firing and K^+ currents were attenuated by intracellular phalloidin, an indication that these effects are mediated through actin disruption.
Description:
The effects of latrunculin A, isolated from the nudibranch Chromodoris sp., on the excitability of neonatal rat cultured dorsal root ganglion neurones were investigated using patch-clamp recording and Ca^2^+ imaging techniques. Under current-clamp conditions, acute application of latrunculin A (100 @mM) reversibly induced multiple action potential firing and significantly increased action potential duration. No significant effects on action potential peak amplitude, threshold of action potential firing, resting membrane potential and input resistance were observed. Under voltage-clamp conditions, significant and dose-dependent suppression of K^+ current was seen with 10-100 @mM latrunculin A. Additionally, a significant difference between inhibition of the current measured at the peak and the end of a 100 ms voltage step was seen with 100 @mM latrunculin A. Fura-2 fluorescence Ca^2^+ imaging revealed that latrunculin A (100 @mM) significantly inhibited Ca^2^+ transients evoked by KCl-induced depolarisation in all neurones. In 36% of DRG neurones, latrunculin A alone had no effect on intracellular Ca^2^+. In 64% of neurones, latrunculin A alone evoked a transient rise in intracellular Ca^2^+. Moreover, latrunculin A (10-100 @mM) significantly inhibited the mean high voltage-activated Ca^2^+ current. The effects of latrunculin A on action potential firing and K^+ currents were attenuated by intracellular phalloidin, an indication that these effects are mediated through actin disruption.
