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Analysis of isoproturon at trace level by solid phase competitive fluoroimmunosensing after enrichment in a sol-gel immunosorbent [An article from: Analytica Chimica Acta]
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PublisherElsevier
ISBN / ASINB000RR71QC
ISBN-13978B000RR71Q0
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This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Isoproturon was extracted selectively from environmental materials (water samples) using an immunosorbent column containing anti-isoproturon antibodies encapsulated in a silica matrix by a sol-gel process. A phosphate buffered saline (PBS) conditioned immunosorbent column was used to on-line preconcentrate 5ml well and tap water containing 0.05@mgl^-^1 of isoproturon, which were desorbed with 75@ml of citric acid and determined with a solid phase competitive fluoroimmunoassay. The solid phase of the immunosensor, consisting of a sol-gel glass doped with anti-isoproturon monoclonal antibody, was placed on the flow-cell of the spectrofluorometer. Free isoproturon in solution competed with a fluorescent conjugated isoproturon and reduced the support bonded fluorescence in a concentration-dependent manner. The on-line method has a detection limit of 9.7ngl^-^1, relative standard deviation of 4 and 3% for 0.05 and 0.5@mgl^-^1, respectively, and recoveries higher than 90% for tap and well water. For comparison the off-line extraction and clean up using a C18 cartridge is also reported.
Description:
Isoproturon was extracted selectively from environmental materials (water samples) using an immunosorbent column containing anti-isoproturon antibodies encapsulated in a silica matrix by a sol-gel process. A phosphate buffered saline (PBS) conditioned immunosorbent column was used to on-line preconcentrate 5ml well and tap water containing 0.05@mgl^-^1 of isoproturon, which were desorbed with 75@ml of citric acid and determined with a solid phase competitive fluoroimmunoassay. The solid phase of the immunosensor, consisting of a sol-gel glass doped with anti-isoproturon monoclonal antibody, was placed on the flow-cell of the spectrofluorometer. Free isoproturon in solution competed with a fluorescent conjugated isoproturon and reduced the support bonded fluorescence in a concentration-dependent manner. The on-line method has a detection limit of 9.7ngl^-^1, relative standard deviation of 4 and 3% for 0.05 and 0.5@mgl^-^1, respectively, and recoveries higher than 90% for tap and well water. For comparison the off-line extraction and clean up using a C18 cartridge is also reported.
