Protective effect of ferulic acid on @c-radiation-induced micronuclei, dicentric aberration and lipid peroxidation in human lymphocytes [An article from: ... Toxicology and Environmental Mutagenesis]

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Description:
In this study we examined radioprotective effect of ferulic acid (FA) on gamma radiation-induced dicentric aberration and lipid peroxidation with reference to alterations in cellular antioxidant status in cultured lymphocytes. To establish most effective protective support we used three different concentrations of FA (1, 5 and 10@mg/ml) and three different doses of @c-radiation (1, 2 and 4Gy). Treatment of lymphocytes with FA alone (at 10@mg/ml) gave no significant change in micronuclei (MN), dicentric aberration (DC), thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) or glutathione peroxidase (GPx) activities when compared with normal lymphocytes; irradiation at 1, 2 and 4Gy increased the MN and DC frequencies in a dose-dependent manner. Treatment with FA for 30min before radiation exposure resulted in a significant decline of MN and DC yields as FA concentration increased. Compared to 1Gy exposure alone, the extent to which FA (1@mg/ml) reduced the MN and DC yields was 75% and 50%, respectively. With 4Gy irradiation, FA (10@mg/ml) decreased 45% MN and 25% DC frequencies. FA-pretreated lymphocytes (1, 5 and 10@mg/ml) showed progressively decreased TBARS levels after irradiation. Irradiation (1, 2 and 4Gy) significantly decreased GSH levels, SOD, CAT and GPx activities in a dose-dependent manner. Pretreatment with 10@mg/ml of FA significantly (p