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Cadmium and zinc induction of ZnT-1 mRNA in an established carp cell line [An article from: Comparative Biochemistry and Physiology, Part C]
Book Details
PublisherElsevier
ISBN / ASINB000RR8FZS
ISBN-13978B000RR8FZ8
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Description
This digital document is a journal article from Comparative Biochemistry and Physiology, Part C, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
The cDNA of a zinc transporter-1 (ZnT-1) gene was cloned from an established cell line derived from common carp (Cyprinus carpio) using rapid amplification of cDNA ends (RACE). Using real-time quantitative PCR, we showed that both zinc (Zn) and cadmium (Cd) transiently upregulate ZnT-1 mRNA to comparable levels. The loosely bound cellular Zn pool, as estimated using the Zn-specific probe FluoZin-3, was increased threefold after exposure to 250@mM ZnCl"2. Correspondingly, the ZnT-1 mRNA level at 24h was induced about fivefold, reflecting the need for more zinc export capacity. Total cellular zinc levels were not different from the controls after 72h of exposure to 10, 50, or 250@mM ZnCl"2. A loss of total cellular Zn but little labile zinc changes were observed with up to 25@mM Cd. At 72h, the total Zn was partially restored to the control levels, only 1@mM Cd allowed for a full recovery. Downregulation of ZnT-1 mRNA and partial loss of loosely bound Zn were observed with 50@mM Cd. Our results clearly show that although Zn and Cd can both regulate Zn export in EPC cells, the effects on the cellular Zn pools are quite different.
Description:
The cDNA of a zinc transporter-1 (ZnT-1) gene was cloned from an established cell line derived from common carp (Cyprinus carpio) using rapid amplification of cDNA ends (RACE). Using real-time quantitative PCR, we showed that both zinc (Zn) and cadmium (Cd) transiently upregulate ZnT-1 mRNA to comparable levels. The loosely bound cellular Zn pool, as estimated using the Zn-specific probe FluoZin-3, was increased threefold after exposure to 250@mM ZnCl"2. Correspondingly, the ZnT-1 mRNA level at 24h was induced about fivefold, reflecting the need for more zinc export capacity. Total cellular zinc levels were not different from the controls after 72h of exposure to 10, 50, or 250@mM ZnCl"2. A loss of total cellular Zn but little labile zinc changes were observed with up to 25@mM Cd. At 72h, the total Zn was partially restored to the control levels, only 1@mM Cd allowed for a full recovery. Downregulation of ZnT-1 mRNA and partial loss of loosely bound Zn were observed with 50@mM Cd. Our results clearly show that although Zn and Cd can both regulate Zn export in EPC cells, the effects on the cellular Zn pools are quite different.
