![Quantification of 5'-monophosphate cytosine arabinoside (Ara-CMP) in cell extracts using liquid chromatography-electrospray mass spectrometry [An article from: Analytica Chimica Acta]](https://www.ebooknetworking.net/books/B00/0RR/bigB000RR8J1S.jpg)
Quantification of 5'-monophosphate cytosine arabinoside (Ara-CMP) in cell extracts using liquid chromatography-electrospray mass spectrometry [An article from: Analytica Chimica Acta]
Description
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
A LC/MS/ESI method is described to monitor 5'-monophosphate cytosine arabinoside (Ara-CMP) formed after incubation of an Ara-C prodrug in cell extracts. After addition of an internal standard and off-line treatment with perchloric acid, Ara-CMP is separated from Ara-C and endogeneous matrix components on a porous graphitic carbon stationary phase using gradient elution with a water/acetonitrile mobile phase containing formic acid. The results of method performances (accuracy, linearity, limit of quantification and repeatability) show that the method is acceptable for its intended use.
Description:
A LC/MS/ESI method is described to monitor 5'-monophosphate cytosine arabinoside (Ara-CMP) formed after incubation of an Ara-C prodrug in cell extracts. After addition of an internal standard and off-line treatment with perchloric acid, Ara-CMP is separated from Ara-C and endogeneous matrix components on a porous graphitic carbon stationary phase using gradient elution with a water/acetonitrile mobile phase containing formic acid. The results of method performances (accuracy, linearity, limit of quantification and repeatability) show that the method is acceptable for its intended use.
