A simple and rapid method for identification and determination of cordycepin in Cordyceps militaris by capillary electrophoresis [An article from: Analytica Chimica Acta]

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Description:
Cordycepin is the main active metabolite of Cordyceps militaris extracts; according to recent studies it has interesting therapeutic activities. A new capillary electrophoresis (CE) procedure with UV detection at 254nm for determination of cordycepin was developed and optimized. Optimal conditions found were 20mM sodium borate buffer with 28.6% methanol, pH 9.5, separation voltage 20kV, hydrodynamic injection time 10s and temperature 25^oC. Linearity was found over the 20-100@mg/mL concentration ranges of cordycepin. The developed method has been applied for determination of cordycepin in various pharmaceutical products. A comparison was made between CE and a high performance liquid chromatography (HPLC) method. Both of these methods gave comparable results. The shorter analysis time and low running cost are the main advantages of CE method.