Directly light scattering imaging of the aggregations of biopolymer bound chromium(III) hydrolytic oligomers in aqueous phase and liquid/liquid interface [An article from: Analytica Chimica Acta]

This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
Investigations of inorganic oligomers are important in both chemistry and physiology. In this contribution, we propose a laser induced light scattering imaging (LSI) and a total internal reflected light scattering imaging (TIR-LSI) technique, and apply them to characterize the interactions of inorganic oligomers with biopolymer in aqueous phase and at liquid/liquid interface, respectively. In aqueous medium, synthetic chromium(III) hydrolytic oligomers (CrHO) react with DNA, and the resultant binary could be extracted into the H"2O/CCl"4 interface in the presence of triocyctyl phosphine oxide (TOPO), forming a DNA-CrHO-TOPO ternary amphipathic complex at the interface with the associate constant of 1.32x10^3mol^-^1dm^4 for a given 1.0x10^-^4moll^-^1 TOPO. Under the excitation of a 441-nm He-Cd laser light beam, the resultant light scattering and total internal reflected light scattering (TIR-LS) signals of the formed binary in aqueous phase and ternary at liquid/liquid interface could be easily captured using a common microscope coupled with a CCD camera. By digitally analyzing the CCD captures, we demonstrate that aggregations of the CrHO-DNA binary in aqueous phase and DNA-CrHO-TOPO ternary at liquid/liquid interface have occurred, respectively.