Simultaneous determination of five quinoxaline-1,4-dioxides in animal feeds using ultrasonic solvent extraction and high-performance liquid chromatography [An article from: Analytica Chimica Acta]

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Description:
An HPLC method with UV detection has been established for the simultaneous quantitative determination of five quinoxaline-1,4-dioxides (carbadox, olaquindox, cyadox, mequindox, quinocetone) in porcine, chicken and fish feeds. Feed samples were extracted with methanol/acetonitrile/water (35:35:30, v/v/v) in an ultrasonic bath, and purified by solid phase extraction (SPE) on Alumina N cartridges. The samples were analyzed on an Eclipse XDB C"1"8 liquid chromatography column using a gradient program with methanol and water. Except for cyadox (>75%), recoveries of the drugs from feed samples spiked at 5, 50 and 200mgkg^-^1 ranged from 92 to 104%. Coefficients of variation were 2-13%. The decision limits (CC@a) for the five compounds were