Isolation of free-living dinitrogen-fixing bacteria and their activity in compost containing de-inking paper sludge [An article from: Bioresource Technology]

This digital document is a journal article from Bioresource Technology, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
Knowledge of the microbiology of dinitrogen (N"2)-fixing bacteria in compost rich in de-inking paper sludge (DPS) is limited. Dinitrogen (N"2)-fixing bacteria from DPS composts were isolated and studied for their N"2-fixing activity in vitro and in vivo. Two Gram-negative N"2-fixing isolates were identified as Pseudomonas. At 20^oC, both isolates revealed that N"2-fixing activity was higher than that of three arctic Pseudomonas strains. Their N"2-fixing activity was found to occur between 18 and 25^oC, a pattern that was similar to the reference isolate Azotobacter ATCC 7486. Composts successfully showed N"2-fixing activity after carbohydrate amendments both with and without inoculation of a N"2-fixing isolate. These results suggest that DPS composts support N"2-fixing bacteria and that N"2-fixing activity is dependent on a usable carbohydrate source.