The protons of gluconic acid are the major factor responsible for the dissolution of tricalcium phosphate by Burkholderia cepacia CC-Al74 [An article from: Bioresource Technology]

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Description:
Burkholderia cepacia CC-Al74 with a high ability for solubilizing tricalcium phosphate (TCP) was used to study the P-solubilization mechanism. We collected filtrates able to solubilize TCP from the cultures of strain CC-Al74 and demonstrated that the P-solubilization increased from 0@mgml^-^1 to 200@mgml^-^1 during exponential growth, when the pH decreased from 8 to 3. HPLC-analysis revealed that the solubilization of TCP was mainly caused by the release of 16.3mM gluconic acid. At this concentration, gluconic acid was capable of solubilizing 376@mgml^-^1 of TCP whereas water at pH 3 only solubilized 35@mgml^-^1. The difference is due to the final H^+ concentrations which were 13.5mM and 1mM in 16.3mM gluconic acid and deionized water, respectively at pH 3.