Bacteria Transformation of Escherichia Coli
Book Details
Author(s)Keelie Smith
ISBN / ASINB008B0TFXO
ISBN-13978B008B0TFX4
Sales Rank2,432,602
MarketplaceUnited States 🇺🇸
Description
This experiment allowed me to learn how to transform the genetic makeup of bacteria, and I could apply it to other organisms. This lab was done to increase the understanding of how plasmids work. The purpose of this lab is to transform the genetic makeup of Escherichia coli using heat and Luria Broth (LB) + or – and Ampicillin so that the plasmid that contains an enzyme that makes E. coli resistant to Ampicillin will be expressed.
In order to see whether or not the bacteria has the plasmid, pGreen will be used so the bacteria will be visible under the black light, only the bacteria with the plasmid will glow because it will be the plasmid that picks up the pGreen. If there is Ampicillin with the Luria Broth, the E. coli will not grow because it is an antibiotic. If there is no plasmid in the E. coli, the Ampicillin will stop any of the bacteria from growing. The reason for some of the E. coli being able to grow when the Ampicillin is used, and there is a plasmid present, is due to the enzyme in the plasmid called beta lactimase. This enzyme disables the antibiotic. If a plasmid is present in the E. coli, the ampicillin will not be able to stop its growth, because the plasmid contains the beta lactimase enzyme, which blocks the antibiotic from binding to the bacteria. The independent variables are whether there is ampicillin and a plasmid present in the test. The dependent variable is the amount of bacteria grown.
This experiment showed how bacteria can obtain foreign DNA, and use it as its own. This often happens when a bacteria needs to be resistant to predators such as antibiotics.
Vibrio cholerae is an important human pathogen and the cause of cholera. Since genetic variation and antibiotic resistance of strains have implications for effective treatment of the disease, we examined the genetic diversity and antibiotic resistance profile in 92 clinical strains (serogroup O1) and 56 environmental strains. (Campos et al.)
This shows that vibrio choreae causes cholera, and though there have been many attempts to stop this pathogen from causing disease, it has used its genetic variation to become resistant to the antibiotics. This emphasizes how genetic variation is essential for bacteria to overcome predators.
In order to see whether or not the bacteria has the plasmid, pGreen will be used so the bacteria will be visible under the black light, only the bacteria with the plasmid will glow because it will be the plasmid that picks up the pGreen. If there is Ampicillin with the Luria Broth, the E. coli will not grow because it is an antibiotic. If there is no plasmid in the E. coli, the Ampicillin will stop any of the bacteria from growing. The reason for some of the E. coli being able to grow when the Ampicillin is used, and there is a plasmid present, is due to the enzyme in the plasmid called beta lactimase. This enzyme disables the antibiotic. If a plasmid is present in the E. coli, the ampicillin will not be able to stop its growth, because the plasmid contains the beta lactimase enzyme, which blocks the antibiotic from binding to the bacteria. The independent variables are whether there is ampicillin and a plasmid present in the test. The dependent variable is the amount of bacteria grown.
This experiment showed how bacteria can obtain foreign DNA, and use it as its own. This often happens when a bacteria needs to be resistant to predators such as antibiotics.
Vibrio cholerae is an important human pathogen and the cause of cholera. Since genetic variation and antibiotic resistance of strains have implications for effective treatment of the disease, we examined the genetic diversity and antibiotic resistance profile in 92 clinical strains (serogroup O1) and 56 environmental strains. (Campos et al.)
This shows that vibrio choreae causes cholera, and though there have been many attempts to stop this pathogen from causing disease, it has used its genetic variation to become resistant to the antibiotics. This emphasizes how genetic variation is essential for bacteria to overcome predators.
