The Journal of Experimental Zoology, Vol. 17 (Classic Reprint)

Nevertheless, a certain degree of simplicity may often be obtained in the conditions of experimentation and yet coincide with the usual conditions of a varied environment to a sufficient degree so that the results give a definite answer to questions which are directed toward finding out what the principles are which underlie the processes. The following experiments have been carried out with an attempt to fulfill the above requirements. In a previous paper (L und 14) some of the most important external relations of Bursaria to food have been presented, including a demonstration of a selective extrusion among the food vacuoles. The following paper deals with the processes which take place in the food vacuole, and with the conditions under which it exists as an active system in this unicellular animal. .P roteins, fats, and carbohydrates in the form of starches are present in the material eaten by Bursaria, as may easily be detected by direct observation or by the aid of microchemical tests. Protein in the form of other living ciliates, flagellates, etc., is taken in and digested. Many of these Protozoa which serve as food contain fat globules and starch or amylum grains, and hence all three food substances may at times be found present in the cytoplasm of Bursaria from flourishing cultures. Since there is disagreement between results of different investigators, working with the same I nfusoria; but more particularly because there are indications that similar forms among these animals differ in their food metabolism, the statements here made refer only to Bursaria and may or may not be valid for others, except where specifically stated.
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